36 pp. Ceratitis capitata eggs and pupae have not been tested. A C. capitata specimen is identified when the PCR product is digested as follows: DraI: 400, 350, 170 and 100 bp, HinfI: 900 and 120 bp, SspI: 510, 230, 150, 100 and 30 bp and TaqI: 480, 470 and 70 bp. United States Department of Agriculture Bulletin 640: 1-43. DraI, HinfI, SspI and TaqI (10 U μL−1, Promega) used for amplicon digestion at a final amount of five Units. The older version of the trap used a protein bait that captured large numbers of non-target insects. CAB International. A female medfly will lay one to 10 eggs in an egg cavity 1 mm deep, may lay as many as 22 eggs per day, and may lay as many as 800 eggs during her lifetime (usually about 300). Figure 18. 4) and by the apical half of the scutellum being entirely black (Fig. The pharyngeal skeleton is distinctive in overall configuration, particularly the enlarged subhypostomium of the hypostomium (posterior to each mouth hook). Wings, usually held in a drooping position on live flies, are broad and hyaline with black, brown, and brownish yellow markings. Females can be distinguished by the characteristic wing pattern (Fig. Figure 24. The anterior of the dorsal bridge has a prominent sclerotized point. Larva of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann). Use of brand names of chemicals or equipment in these EPPO Standards implies no approval of them to the exclusion of others that may also be suitable. Journal of Economic Entomology 64: 708-713. Method validation of the ITS1 PCR‐RFLP assay for the identification of adults and larvae of Ceratitis capitata has been performed according to the Dutch national guideline for the validation of detection and identification methods for plant pathogens and pests (Version 2, March 2010) which is based on EPPO standard PM7/98. Pupation, adult emergence and sex ratios of survived flies were investigated to study ITS1 primers ITS1‐F5/ITS1‐B9 amplify 1020 bp of C. capitata DNA. Egg: The egg is very slender, curved, 1 mm long, smooth and shiny white. Leftwich PT(1), Nash WJ(1), Friend LA(1), Chapman T(1). It will only separate Ceratitis from the four other major pest genera, and C. capitata from a few other Ceratitis spp. Adaptation to divergent larval diets in the medfly, Ceratitis capitata. During warm weather eggs hatch in 1.5 to three days. United States Department of Agriculture Cooperative Plant Pest Report 1: 117-118. Ceratitis capitata 3 Larva Described by Hardy (1949), Orian & Moutia (1960), Sabatino (1974), Berg (1979), Heppner (1985), Smith (1989), White & Elson-Harris (1992). Wings are usually held in a drooping position on live flies, are broad and hyaline with black, brown, and brownish yellow markings. Females usually die soon after they cease to oviposit. Primers described by Douglas and Haymer (2001) located in the 18S and 5.8S regions spanning the ITS1 region: forward primer ITS1‐F5 (5′‐ CAC GGT TGT TTC GCA AAA GTT G – 3′) and reverse primer ITS1‐B9 (5′‐ TGC AGT TCA CAC GAT GAC GCA C – 3′), each used at a final concentration of 0.4 μM. StephensonB@maf.govt.nz. Primary larval identification characters used: mouth hooks and cephalo‐pharyngeal skeleton, anterior spiracles (Fig. 3). Harvesting before complete maturity also is practiced in Mediterranean areas generally infested with this fruit fly. Photograph by Scott Bauer, USDA. Cooperative Mediterranean Fruit Fly Project (California). Peach infested with larvae of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann). The supposed supernumerary lateral papillules of the caudal end, noted by Phillips (1946), are not usually evident in specimens or at most represent only slight plate elevations on the very lateral edge dorsal to L1. Native to sub‐Saharan Africa, Ceratitis capitata has spread to Mauritius, Reunion, Seychelles, North Africa, Southern Europe, the … The figure of the cephalo-pharyngeal skeleton in Greene (1929) appears not to be very accurate. Sometimes there may be some tissue decay or secondary rot around these marks, and some fruits with a very high sugar content (e.g. 1977. Scutellum yellow‐white basally, apically with three merged black spots (Fig. The improved version of the McPhail trap uses a combination of three chemicals to attract male and female fruit flies. d Pupa: (after Weems, 1981) Cylindrical 4–4.3 mm long, dark reddish brown, resembling Usually about 50% of the flies die during the first two months after emergence. The micropylar region is distinctly tubercular. 1.0 μL extracted DNA obtained as described above. By the third instar, larvae are opaque white and 0.6 to 0.8 cm long. Older version of trap used to capture adults of the Adult Mediterranean fruit fly, Ceratitis capitata (Wiedemann). Drawing by G.J. Ceratitis capitata (Wiedemann, 1824) es un díptero perteneciente a la familia Tephritidae.Es originaria de la costa occidental de África, donde viven especies muy próximas, desde donde se ha extendido a otras zonas templadas, subtropicales y tropicales de los dos hemisferios.Es considerada como especie cosmopolita, por su dispersión debida al transporte de productos realizado por el hombre. When the eggs hatch, the larvae promptly begin eating. Ceratitis capitata is a highly polyphagous species whose larvae develop in a very wide range of unrelated fruits. Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), in apples of the varieties Golden Delicious, Granny Smith and Red Delicious. A female Mediterranean fruit fly, Ceratitis capitata (Wiedemann), pumps eggs through her ovipositor into the soft outer layers of a ripe coffee berry. This protocol was originally drafted by Ms V. Balmès, Laboratoire de la Santé des Végétaux, unité entomologie et plantes invasives‐ CBGP – Campus internationnnal de Baillarguet CS30016‐ 34988 Montferrier sur Lez, France. Two minutes at 94°C, 35 cycles of 1 min at 94°C, 30 s at 63°C, and 1 min at 72°C, followed by a final extension for 10 min at 72°C and quickly cooled to room temperature. The full text of this article hosted at iucr.org is unavailable due to technical difficulties. Journal of Agricultural Research 3: 363-374. The last instar is usually 7 to 9 mm in length, with eight ventral fusiform areas. 2), rear view maggot (posterior view and lateral view of posterior end), and shape and arrangement of caudal spiracles. The Mediterranean fruit fly attacks more than 260 different fruits, flowers, vegetables, and nuts. Larvae of Ceratitis capitata were tested for preference for different diets based on the ingredients of the standard diet used to rear flies in the laboratory. Head is to the left. The ITS1 PCR‐RFLP results obtained using the C. capitata specimens were consistent and unique to target species. The shape of the interior sclerotizations of the dorsal wing plate and the hood of the pharyngeal plate are also distinctive for the species. Use of brand names of chemicals or equipment in these EPPO Standards implies no approval of them to the exclusion of others that may also be suitable. Dorsocentral bristles are anterior of the halfway point between supraalar and acrostichal bristles. Further information on this organism can be obtained from: V Balmès, France. Steck and B.D. Joint cooperative Mediterranean fruit fly eradication project. Larvae of Ceratitis capitata. Please note that morphological terminology follows White & Elson‐Harris (1992). Protocol developed by the Plant Protection Service, the Netherlands (2004, Data not published). Figure 20. Ceratitis capitata Ceratitis capitata 2011-12-01 00:00:00 Introduction Ceratitis capitata is the most serious pest for citrus and many other fruits in the majority of countries with a warm, Mediterranean, tropical or subtropical climate ( EPPO/CABI, 1997 ). Journal of Agricultural Research 38: 489-504. Plant Protection and Quarantine Programs, Animal and Plant Health Inspection Service, United States Department of Agriculture. Despite the importance of bacteria on larval development, very little is known about the interaction between bacteria and larvae in their true ecological context. Adults emerge in largest numbers early in the morning during warm weather and emerge more sporadically during cool weather.